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Posters & Publications

Posters and Publications

A Novel Urine Exosome Gene Expression Assay to Predict High-grade Prostate Cancer at Initial Biopsy

Published in JAMA Oncology, March 2016 (ePrint download coming soon)
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Association of plasma exosomal mRNA changes with long-term durable response to ipilimumabin metastatic melanoma patients

Presented at: 2016 American Society of Clinical Oncology Annual Meeting (Chicago; June 2016)
Authors: C. Coticchia, R. Sullivan, J. Hurley, D. Lawrence, K. Flaherty, S. Blackmon, G. Brock and J. Skog
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Discovery and validation of a urinary exosome mRNA signature for the diagnosis of human kidney transplant rejection

Presented at: Annual Meeting of International Society for Extracellular Vesicles (Rotterdam, the Netherlands; May 2016)
Authors: J. Hurley, S. Bentink, L. Hu, A. Srivastava, E. Karecci, A.Mihali, D. Gauvin, G. Brock, J. Azzi and J. Skog
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Exosomal RNA based liquid biopsy detection of androgen receptor variant 7 in plasma from prostate cancer patients

Presented at: 2016 American Association for Cancer Research Annual Meeting (New Orleans, LA; April 2016)
Authors: J. Hurley, G. Brock, J. Siddiqui, Rohit Malik, Arul Chinnaiyan, J. Skog
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Exosomal RNA-based liquid biopsy detection of EML4-ALK in plasma from NSCLC patients

Presented at: 2016 National Comprehensive Cancer Network 21st Annual Conference (Hollywood, FL; March 2016)
Authors: K. Brinkmann, J. Emenegger, D. Carbone, J. Hurley, E. Castellanos-Rizaldos, D. Enderle, T. Koestler, A. Spiel, R. Mueller, D. Baughman, G. Brock, V. O’Neill, J. Skog
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Extended analysis of a validated urine-exosome signature to predict high grade prostate cancer on initial biopsy maintains performance across multiple sub-groups

Presented at: 2016 Genitourinary Cancers Symposium, (San Francisco, CA; January 2016)
Authors: M. Donovan, S. Bentink, K, M. Noerholm, V. O’Neill, J. Skog
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A non-invasive urine exosome gene expression assay (ExoIntelliScoreTM Prostate) accurately predicts pathological stage and grade in the prostatectomy specimen

Presented at: 2016 Genitourinary Cancers Symposium, (San Francisco, CA; January 2016)
Authors: M. Donovan, J. Eastham, V. Patel, J. McKiernan, M. Noerholm, S. Belzer, S. Bentink, J. Skog, V. O’Neill
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Early exosome mRNA changes are associated with improved progression free survival of metastatic melanoma patients on ipilimumab: Identification of a novel exosome mRNA signature of ipilimumab response

Presented at: 26th AACR-NCI-EORTC International Conference on Molecular Targets and Cancer Therapeutics, (Boston, Mass.; November 2015)
Authors: C. Coticchia, R. Sullivan, K. Flaherty, J. Hurley, L. Hu, S. Blackmon, G. Brock, V. O’Neill, J. Skog
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Plasma EGFR mutation detection using a combined exosomal RNA and circulating tumor DNA approach in patients with acquired resistance to first-generation EGFR-TKIs

Presented at: 26th AACR-NCI-EORTC International Conference on Molecular Targets and Cancer Therapeutics, (Boston, Mass.; November 2015)
Authors: A.K. Krug, C. Karlovich, T. Koestler, S. Bentink, K. Brinkmann, A. Spiel, J. Emenegger, M. Noerholm, V. O'Neill, L.V. Sequist, JC. Soria, J.W. Goldman, D. Ross Camidge, H.A. Wakelee, S.M. Gadgeel, E. Mann, S. Matheny, L. Rolfe, M. Raponi, D. Enderle, J. Skog
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Profiling exosomal mRNAs in patients undergoing immunotherapy for malignant melanoma

Presented at: 18th European Cancer Congress, (Vienna, Austria; September 2015) 
Authors: M. Donovan, J. Eastham, V. Patel, J. McKiernan, M. Noerholm, S. Belzer, S. Bentink, V. O'Neill
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A non-invasive urine exosome gene expression assay (ExoIntelliScore™ Prostate) accurately predicts pathologic stage and grade in the prostatectomy specimen

Presented at: 18th European Cancer Congress, (Vienna, Austria; September 2015)
Authors: J. Hurley, L. Hu, G. Brock, I. Sinclair, R. Sullivan, V. O'Neill, J. Skog, K. Flaherty
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Exosomal RNA-based liquid biopsy detection of EML4-ALK in plasma from NSCLC patients

Presented at: 2015 IASLC 16th World Conference on Lung Cancer, (Denver, Colorado; September 2015)
Authors: K. Brinkmann, J. Emenegger, D. Carbone, J. Hurley, E. Castellanos-Rizaldos, D. Enderle, T. Koestler, A. Spiel, R. Mueller, G. Brock, V. O’Neill, J. Skog, M. Noerholm 
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EGFR activating and T790M resistance mutation in plasma exoRNA and cfDNA, detected with single-step isolation columns and targeted resequencing

Presented at: 2015 IASLC 16th World Conference on Lung Cancer, (Denver, Colorado; September 2015)
Authors: A.K. Krug, T. Koestler, D. Enderle, K. Brinkmann, A. Spiel, J. Emenegger, J.J. Wheler , R. Mueller, G. Stoll, S. Bentink, F. Janku, J.Skog, M. Noerholm, V. O’Neill
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Detection of EGFR activating and T790M resistance mutation in plasma of NSCLC patients using combined exosomal RNA and cfDNA capture

Presented at: 16th Annual International Lung Cancer Congress (Huntington Beach, California; July 2015)
Authors: V. J. O’Neill, K. Brinkmann, D. Enderle, T. Koestler, J. Emenegger, A. Spiel, J. Skog, M. Noerholm
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Plasma-Based Monitoring of BRAF Mutations During Therapy for Malignant Melanoma Using Combined Exosomal RNA and Cell-Free DNA Analysis

Presented at: 2015 American Society of Clinical Oncology Annual Meeting (Chicago, Illinois; May 2015)
Authors: R. J. Sullivan, V. J. O’Neill, D. Enderle, M. Valentino, T. Koestler, A. Spiel, S. Blackmon, A. K. Krug, K. Brinkmann, S. Bentink, R. Mueller, J. Emenegger, M. Noerholm, J. Skog, C. Berking, K. Flaherty
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Highly sensitive detection of low abundant somatic mutations in circulating exosomal RNA and cfDNA with next-generation sequencing

Presented at: 2015 American Society of Clinical Oncology Annual Meeting (Chicago, Illinois; May 2015)
Authors: D. Enderle, A. K. Krug, T. Koestler, A. Spiel, J. J. Wheler, K. Brinkmann, J. Emenegger, M. Valentino, S. Bentink, R. Mueller, R. J. Sullivan, K. Flaherty, C. Berking, F. Janku, J. Skog, M. Noerholm, V. J. O’Neill
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Interim performance of a non-DRE urine exosome gene signature to predict Gleason ≥7 prostate cancer on initial prostate needle biopsy from patients enrolled in a prospective observational trial

Presented at: 2015 American Society of Clinical Oncology Annual Meeting (Chicago, Illinois; May 2015)
Authors: M. J. Donovan, M. Noerholm, S. Bentink, S. Belzer, J. Skog, G. A. Brown, J. S. Cochran, V. O'Neill
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Validation of a Novel Non-Invasive Urine Exosome Gene Expression Assay to Predict High-grade Prostate Cancer in Patients Undergoing Initial Biopsy with an Equivocal PSA

Presented at: 2015 American Urological Association Annual Meeting
Presenter: Dr. James McKiernan
View plenary late-breaking abstract presentation.

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Plasma-based diagnostics for detection of EML4-ALK fusion transcripts in NSCLC patients

Presented at: 2015 American Association for Cancer Research Annual Meeting (Philadelphia, PA; April 2015)
Authors: K. Brinkmann, D. Enderle, T. Koestler, S. Bentink, J. Emenegger, A. Spiel, R. Mueller, V. O’Neill, J. Sko2, D. Carbone, M. Noerholm
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A first catch, non-DRE urine exosome gene signature to predict Gleason 7 prostate cancer on an initial prostate needle biopsy

Presented at: 2015 Genitourinary Cancers Symposium (Orlando, Florida; February 2015)
Authors: M. J. Donovan, M. Noerholm, S. Bentink, S. Belzer, J. Skog, G. A. Brown, J. S. Cochran, V. O'Neill
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Development of a single-step isolation platform to analyze exosomal RNA and cell-free DNA in plasma from cancer patients

Presented at: 26th EORTC-NCI-AACR Symposium on Molecular Targets and Cancer Therapeutics (Barcelona, Spain; November 2014)
Authors: D. Enderle, T. Koestler, A. Spiel, K. Brinkmann, S. Bentink, J. Skog, M. Noerholm
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Monitoring therapy response and resistance mutations in circulating RNA and DNA of plasma from melanoma patients

Presented at: 26th EORTC-NCI-AACR Symposium on Molecular Targets and Cancer Therapeutics (Barcelona, Spain; November 2014)
Authors: D. Enderle, T. Koestler, R.J. Sullivan, A. Spiel, K. Brinkmann, R. Mueller, S. Bentink, V. O’Neill, J. Skog, M. Noerholm, C. Berking, K.T. Flaherty
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Circulating miRNAs in Human Plasma

Presented at: The International Society for Extracellular Vesicles (ISEV) Annual Meeting (Rotterdam; April 2014)
Authors: Daniel Enderle, Alexandra Spiel, Tina Koestler, Martin Schlumpberger, Markus Sprenger-Haussels, Jonathan Shaffer, Eric Lader, Johan Skog, Mikkel Noerholm
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Characterization of RNA from extracellular vesicles

Presented at: The International Society for Extracellular Vesicles (ISEV) Annual Meeting (Rotterdam; April 2014)
Authors: Daniel Enderle, Alexandra Spiel, Romy Mueller, Charlotte Romain, Emily Berghoff, Susan Belzer, Stefan Bentink, Martin Schlumpberger, Markus Sprenger-Haussels, Jonathan Shaffer, Eric Lader, Johan Skog, Mikkel Noerholm
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The exoRNeasy Serum/Plasma Maxi Kit for Isolation of RNA from Extracellular Vesicles

Presented on January 30, 2014
Authors: Daniel Enderle, Alexandra Spiel, Romy Müller, Charlotte Romain, Emily Berghoff, Susan Belzer, Tina Koestler, Stefan Bentink, Martin Schlumpberger, Johan Skog, Mikkel Noerholm
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Original Publications

Characterization of RNA from Exosomes and Other Extracellular Vesicles Isolated by a Novel Spin Column-Based Method

PLoS ONE 10(8): e0136133; Published online: 28 August 2015
doi: 10.1371/journal.pone.0136133
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0136133 
Authors: Daniel Enderle, Alexandra Spiel, Christine M. Coticchia, Emily Berghoff, Romy Mueller, Martin Schlumpberger, Markus Sprenger-Haussels, Jonathan, M. Shaffer, Eric Lader, Johan Skog, Mikkel Noerholm
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Liquid biopsy for cancer screening, patient stratification and monitoring

Translational Cancer Research, Vol 4, No 3 (June 2015): 280-290
doi: 10.3978/j.issn.2218-676X.2015.06.05
http://www.thetcr.org/article/view/4546
Authors: Graham Brock, Elena Castellanos-Rizaldos, Lan Hu, Christine Coticchia, Johan Skog
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BEAMing and Droplet Digital PCR Analysis of Mutant IDH1 mRNA in Glioma Patient Serum and Cerebrospinal Fluid Extracellular Vesicles

Molecular Therapy Nucleic Acids (2013) 2, e109 (23 July 2013)
doi: 10.1038/mtna.2013.28
http://www.nature.com/mtna/journal/v2/n7/full/mtna201328a.html
Authors: Walter W Chen, Leonora Balaj, Linda M. Liau, Michael L. Samuels, Steve K. Kotsopoulos, Casey A. Maguire, Lori LoGuidice, Horacio Soto, Matthew Garrett, Lin Dan Zhu, Sarada Sivaraman, Clark Chen, Eric T Wong, Bob S Carter, Fred H Hochberg, Xandra O. Breakefield and Johan Skog
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RNA expression patterns in serum microvesicles from patients with glioblastoma multiforme and controls

BMC Cancer 2012, 12:22
doi: 10.1186/1471-2407-12-22
Authors: Mikkel Noerholm, Leonora Balaj, Tobias Limperg, Afshin Salehi, Lin Dan Zhu, Fred H Hochberg, Xandra O Breakefield, Bob S Carter and Johan Skog
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Melanoma exosomes educate bone marrow progenitor cells toward a pro-metastatic phenotype through MET

Nature Medicine (2012); Published online: 27 May 2012
doi: 10.1038/nm.2753
http://www.nature.com/nm/journal/vaop/ncurrent/full/nm.2753.html
Authors: Héctor Peinado, Maša Alečković, Simon Lavotshkin, Irina Matei, Bruno Costa-Silva, Gema Moreno-Bueno, Marta Hergueta-Redondo, Caitlin Williams, Guillermo García-Santos, Cyrus M Ghajar, Ayuko Nitadori-Hoshino, Caitlin Hoffman, Karen Badal, Benjamin A Garcia, Margaret K Callahan, Jianda Yuan, Vilma R Martins, Johan Skog, Rosandra N Kaplan, Mary S Brady, Jedd D Wolchok, Paul B Chapman, Yibin Kang, Jacqueline Bromberg and David Lyden
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Tumour microvesicles contain retrotransposon elements and amplified oncogene sequences

Nature Communications 2:180 (2011); Published online: 1 February 2011
doi: 10.1038/ncomms1180
http://www.nature.com/ncomms/journal/v2/n2/full/ncomms1180.html
Authors: Leonora Balaj, Ryan Lessard, Lixin Dai, Yoon-Jae Cho, Scott L. Pomeroy, Xandra O. Breakefield and Johan Skog
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Nucleic acids within urinary exosomes/microvesicles are potential biomarkers for renal disease

Kidney International 78, 191–199 (July (2) 2010)
doi: 10.1038/ki.2010.106
http://www.nature.com/ki/journal/v78/n2/full/ki2010106a.html
Authors: Kevin C Miranda, Daniel T Bond, Mary McKee, Johan Skog, Teodor G Păunescu, Nicolas Da Silva, Dennis Brown and Leileata M Russo
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Glioblastoma microvesicles transport RNA and proteins that promote tumour growth and provide diagnostic biomarkers

Nature Cell Biology 10, 1470–1476 (2008); Published online: 16 November 2008
doi: 10.1038/ncb1800
http://www.nature.com/ncb/journal/v10/n12/abs/ncb1800.html
Authors: Johan Skog, Tom Würdinger, Sjoerd van Rijn, Dimphna H. Meijer, Laura Gainche, William T. Curry, Jr., Bob S. Carter, Anna M. Krichevsky and Xandra O. Breakefield
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